Dr. Sanjit Kumar

ASSOCIATE PROFESSOR

Work Experience

March2023- Present: Associate Professor, Department of Biotechnology GGV, Bilaspur, Chhattisgarh January2023-Feb2023: Associate Professor, Centre for Bio-separation and Technology, VIT-Vellore March2014-December2022: Assistant Professor, Centre for Bio-separation and Technology,VIT-Vellore Feb 2013 –Feb2014: DST-Young Scientist Investigator, NIT, Rourkela, Odisha

Area of Specialization

Structure Biology & Protein Biochemistry  Biological X-ray Crystallography  Protein Biochemistry  Activity and Structures of enzymes  Biochemical characterization of proteins/enzymes  Structure based design of enzyme inhibitor molecules of therapeutic interest for pharmaceutical applications

Projects & Patents

1. Understanding the mechanistic role of adenosine deaminase in purine salvage pathway of Mycobacterium tuberculosis (Role: PI) SERB- Core Research Grant 49.5 Lakhs Approved (2023-2026) 2 Characterization of tRNA Adenosine Deaminase from Pseudomonas aeruginosa. (Role: Co-PI) ICMR New- Delhi 52.0 Lakhs Approved (2023-2026) 3 A simple, rapid and affordable vitamin D diagnostic microfluidic strip. (Role: Co-PI) ICMR New- Delhi 20.4 Lakhs 2022-2025 4 Development and trial of potential inhibitors of Biofilm formation in MDR bacteria: Novel approach to combat nosocomial infections (Role-PI) ICMR New- Delhi 15.0 Lakhs 2021-2024 5 Structure based inhibitors design for Lpx (UDPdiacylglucosaminepyrophosphohydrolase) involved in Gram-negative bacterial lipid A biosynthesis (Role : PI) ICMR New- Delhi 32.0 Lakhs 2019-2023 (Extension) 6 A simple microfluidic diagnostic chip for rapid and sensitive evaluation of Vitamin D deficiency. (Role: Co-PI) DBT New- Delhi 42 .0 Lakhs Completed (2019-2022) 7 A simple and robust microchip to screen post translational modifications in Human Plasma, (Role: Co-PI) SERB- DST 28.0 Lakhs Completed (2019-2022) 8 “Structural and functional characterization of SRrp508’’ CSIR-Gov of India as CO-PI sanction for 2016-2019. CSIR 23.0 lakhs Completed (2016-2019) 9 Structural studies of Plant pathogenesis related proteins (PI) SERB- DST 18.0 Lakhs Completed (2013-2016) 10 In-Silico analysis of various insulin variants(Role-PI) Sekkei (SEKII) Bio Pvt. 3Lakhs Completed 2019

Education

Education: • PhD (All India Institute of Medical Sciences (AIIMS), New-Delhi) • MSc (DAVV Indore)

Achievments/Awards/Honors

Publications

PUBLICATIONS (40) https://scholar.google.com/citations?user=wGxiEdoAAAAJ&hl=en Best Fifteen Author Papers 1. Pal SK, Kumar S*., LpxC (UDP-3-O-(R-3-hydroxymyristoyl)-N-acetylglucosamine deacetylase) inhibitors: A long path explored for potent drug design Int J Biol Macromol. (2022) Dec 21:S0141-8130(22)03090-2. doi: 10.1016/j.ijbiomac.(IF: 8.08) ;; 2. Shukla PK, Bissell JE, Kumar S, Pokhrel S, Palani S, Radmall KS, Obidi O, Parnell TJ, Brasch J, Shrieve DC, Chandrasekharan MB. Structure and functional determinants of Rad6-Bre1 subunits in the histone H2B ubiquitin-conjugating complex Nucleic Acids Res. (2023) Mar 21;51(5):2117-2136. doi: 10.1093/nar/gkad012 (IF: 19.1) 3. Pal SK, Kumar S*., Indole-based LpxC (UDP-3-O-(R-3-hydroxyacyl)-N- acetylglucosaminedeacetylase) inhibitors for Salmonella typhi: rational drug discovery through in silico screening 3 Biotech (2023) Aug;13(8):281. doi:10.1007/s13205-023- 03699-5. Epub 2023 Jul 24 (IF: 2.8) 4. Naik S., Kumar S*., Lectins from plant and algae act as anti-viral against HIV, influenza and coronaviruses, Molecular Biology Reports, (2022) DOI: 10.1007/s11033-022-07854- 8 (IF:2.8) 5. Naik S., Deora N., Pal S.K., Ahmed M.Z., Alqahtani A.S., Shukla P.K., Venkatraman K., Kumar S*., Purification, biochemical characterization, and DPP-IV and alpha-amylase inhibitory activity of Berberine from Cardiospermum halicacabum, Journal of Molecular Recognition, (2022)DOI: 10.1002/jmr.2983 (IF: 2.5) 6. Naik S., Kumar S*., Biochemical Characterization of Lactose Binding Entadin Lectin from Entada rheedii Seeds with Cytotoxic Activity against Cancer Cell Lines, ACS Omega, (2021) DOI: 10.1021/acsomega.0c00577 (IF:4.2) 7. Naik S., Rawat R.S., Khandai S., Kumar M., Jena S.S., Vijayalakshmi M.A., Kumar S*., Biochemical characterisation of lectin from Indian hyacinth plant bulbs with potential inhibitory action against human cancer cells, International Journal of Biological Macromolecules,(2017)Vol:105,Pg.No(1349-1356),DOI:10.1016/j.ijbiomac.2017.07.170 (IF:8.08) 8. Serge N.E., Laurette Blandine M.K., Kumar S*., Extraction, purification, and biochemical characterization of serine protease from leaves of Abrus precatorius, Clerg T., Vijayalakshmi M., (2017) Preparative Biochemistry and Biotechnology, Vol:47, Issue: 10, Pg.No(1016-1024), DOI: 10.1080/10826068.2017.1373289 (IF:3.1) 9. Kumar M, Roy A, Rawat RS, Alok A, Tetala KKR, Biswas NR, Kaur P, Kumar S*.Identification and structural studies of natural inhibitors against SARS-CoV-2 viral RNA methyltransferase (NSP16). (2021) J Biomol Struct Dyn. doi: 10.1080/07391102.2021.1997821. Epub 2021 Nov 12.PMID: 34766876 10. Rawat RS, Kumar S*.Understanding the mode of inhibition and molecular interaction of taxifolin with human adenosine deaminase (2021) J Biomol Struct Dyn doi10.1080/07391102.2021.2006087. Epub 2021 Dec 1.PMID: 34851227(IF:5.3) 11. Savane TS, Kumar S*, Janakiraman VN, Kamalanathan AS, Vijayalakshmi MA. (20116) Molecular insight in the purification of immunoglobulin by pseudobiospecific ligand l-histidine and histidyl moieties in histidine ligand affinity chromatography (HLAC) by molecular docking J Chromatogr B Analyt Technol Biomed Life Sci. 2016 May 15;1021:129-136. (IF:5.3) 12. Thomas J, Kumar S, Satija J. Integrated molecular and quantummechanical approach to identify novel potent natural bioactive compound against O-methyltransferase (nsp16) of SARS-CoV-2 J Biomol Struct Dyn (2023) May.2:1-14. doi: 10.1080/07391102.2023.2206287.( IF: 5.3) 13. Chakraborty D., Ghosh D., Kumar S., Jenkins D., Chandrasekaran N., Mukherjee A.,Nano-diagnostics as an emerging platform for oral cancer detection: Current and emerging trends, Wiley Interdisciplinary Reviews: Nanomedicine and Nanobiotechnology, (2022) DOI: 10.1002/wnan.1830 (IF: 9.4) 14. Jadaun P., Seniya C., Pal S.K., Kumar S., Kumar P., Nema V., Kulkarni S.S., Mukherjee A., Elucidation of Antiviral and Antioxidant Potential of C-Phycocyanin against HIV-1 Infection through In Silico and In Vitro Approaches, (2022) Antioxidants, Vol:11, Issue: 10, DOI: 10.3390/antiox11101942 MA (IF: 7.6) 15. Dwivedi AK, Gurjar V, Kumar S, Singh N.Molecular basis for nonspecificity ofnonsteroidal anti-inflammatory drugs (NSAIDs) (2015) Drug Discov Today. 2015 Jul;20(7):863-73. doi: 10.1016/j.drudis.2015.03.004. Epub 2015 Mar 17. * Corresponding Author